Cerium oxide nanofiber–based electrochemical immunosensor for detection of sepsis in biological fluid

Singh, O.K and Singh, S. G. and et al, . (2021) Cerium oxide nanofiber–based electrochemical immunosensor for detection of sepsis in biological fluid. Journal of Solid State Electrochemistry, 25 (10-11). pp. 2587-2598. ISSN 14328488

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Abstract

Sepsis causes life-threatening complications with the highest burden of death and medical expenses in hospitals worldwide. Despite the progression of targeted therapies for sepsis, the challenge of early diagnosis of sepsis-related biomarkers remains. The analysis of the TNF-α and sTREM-1 in biological fluids provides essential information for effective treatments. In this work, we report developing an electrochemical immunosensor for the rapid detection of TNF-α and sTREM-1 proteins in human plasma samples. First, using the electrospinning process, cerium oxide nanofibers were synthesized. Subsequently, the antibodies corresponding to the targeted proteins are immobilized onto the surface-functionalized working electrodes using NHS/EDC chemistry. The proposed immunosensor’s performance in a biological fluid was assessed using an analytical electrochemistry approach. The limit of detection for the electrochemical immunosensors was 0.51 and 0.41 pg/mL for TNF-α and sTREM-1, respectively, with high selectivity and sensitivity for the use as a point of care device.

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