Md, Azhar Ali and Solanki, P R and Patel, M K and Dhayani, H and Agrawal, V V and John, Renu and Malhotra, B D
(2013)
A highly efficient microfluidic nano biochip based on nanostructured nickel oxide.
Nanoscale, 5 (7).
pp. 2883-2891.
ISSN 2040-3364
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Abstract
We present results of the studies relating to fabrication of a microfluidic biosensor chip based on nickel oxide nanorods (NRs-NiO) that is capable of directly measuring the concentration of total cholesterol in human blood through electrochemical detection. Using this chip we demonstrate, with high reliability and in a time efficient manner, the detection of cholesterol present in buffer solutions at clinically relevant concentrations. The microfluidic channel has been fabricated onto a nickel oxide nanorod-based electrode co-immobilized with cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) that serves as the working electrode. Bare indium tin oxide served as the counter electrode. A Ag/AgCl wire introduced to the outlet of the microchannel acts as a reference electrode. The fabricated NiO nanorod-based electrode has been characterized using X-ray diffraction, Raman spectroscopy, HR-TEM, FT-IR, UV-visible spectroscopy and electrochemical techniques. The presented NRs-NiO based microfluidic sensor exhibits linearity in the range of 1.5-10.3 mM, a high sensitivity of 0.12 mA mM-1 cm-2 and a low value of 0.16 mM of the Michaelis-Menten constant (Km).
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