Nanostructured anatase-titanium dioxide based platform for application to microfluidics cholesterol biosensor

Md, Azahar Ali and Srivastava, S and Solanki, P R and Varun Agrawal, V and John, Renu and Malhotra, B D (2012) Nanostructured anatase-titanium dioxide based platform for application to microfluidics cholesterol biosensor. Applied Physics Letters, 10 (8). pp. 1-6. ISSN 0003-6951

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Abstract

We report results of studies relating to the fabrication of a microfluidics cholesterol sensor based on nanocrystalline anatase-titanium dioxide (ant-TiO 2) film deposited onto indium tin oxide (ITO) glass. The results of response studies (optimized under the flow rate of 30 μl/min) conducted on cholesterol oxidase (ChOx) immobilized onto crystalline ant-TiO 2 nanoparticles (∼27 nm)/ITO microfluidics electrode reveal linearity as 1.3 to 10.3 mM and improved sensitivity of 94.65 μA/mM/cm 2. The observed low value of K m (0.14 mM) indicates high affinity of ChOx to cholesterol. No significant changes in current response of this microfluidics sensor are measured in the presence of different interferents

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IITH Creators:

IITH Creators	ORCiD
John, Renu	https://orcid.org/0000-0003-3254-2472

Item Type:

Article

Additional Information:

We thank Director NPL, New Delhi, India for the facili- ties. We thank Mr. Sandeep Singh, NPL, New Delhi for the AFM studies. Md. Azahar Ali and Saurabh Srivastava are thankful to CSIR, India for the award of Research Fellow- ships. PRS is thankful to the DST for Fast Track Young Sci- entist award. VVA is thankful to CSIR for the Empowered project. We are thankful to Professor A. K. Mulchandani for the providing the microfluidic channels.

Uncontrolled Keywords:

Cholesterol biosensor; Cholesterol oxidase; Current response; High affinity; Interferents; Nano-structured; Nanocrystallines

Subjects:

Biomedical Engineering

Divisions:

Department of Biomedical Engineering

Depositing User:

Team Library

Date Deposited:

11 Nov 2014 04:36

Last Modified:

05 Dec 2017 04:26