Ram, G Hanu Phani and Bansode, Vaibhav and John, Renu
(2018)
Lensless holographic microscope of biological samples.
In: Computational Optical Sensing and Imaging, 25–28 June 2018, Florida, United States.
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Abstract
Lensless holographic microscopy shows high potential to be a practical point of care diagnostic tool due to its unique features like compactness, simple prototype, portability and on-chip operation. In the present work, a deep violet (370nm, 10 nm FWHM) LED at low power is used as a low coherent light source for lensless 3-D holographic imaging of live cells and microparticles. An on-chip Fresnel hologram of the sample is recorded in on-axis geometry on a high resolution CMOS camera and 3-D phase reconstruction has been performed eliminating the twin images using an optimization approach.
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