Ahmed, Mohammed Tanveer
(2016)
Study on protein-protein interaction between scRad51 and Tpa1.
Masters thesis, Indian Institute of Technology Hyderabad.
Abstract
In this study, the interactions between the yeast proteins Tpa1 (involved in direct demethylation repair) and scRad51 (involved in recombination repair) are analysed. The interactions between the bacterial homologs, AlkB and RecA were shown in previous research done in this lab. For this study, the proteins were purified using nickel-affinity columns and the interactions were analysed through FPLC. Even though the interactions between bacterial proteins were shown, the same could not be proved for these proteins.
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