G, Hanu Phani Ram and Pandiyan, V P and John, Renu
(2015)
Low cost microscopy for three dimensional imaging using digital inline holographic principle.
International Journal of Research in Engineering and Technology, 42 (12).
pp. 66-68.
ISSN 2321-7308
Abstract
Optical microscopy is
reached a new level in terms of resolution, 3
-
D imaging capability, flexibility of imaging different samples
which increase imaging complexity and the cost.. Though established labs can afford high
-
end microscopes, it remains a concern
in rural areas where
clinics and patients cannot afford much. Semi
-
portable microscopy based on inline holographic setup is
demonstrated where depth information as 3rd dimension can also be accessed. This setup contains only light emitting diode
(LED), pinhole and charge coup
led device (CCD) camera. Since laser source gives rise to speckle noise and it is also cost
constraint for developing a low cost microscopy, thus it is replaced with incoherent LED source. This setup is also known as
‘lensless holography’ because there is
no use of lens for imaging. In conventional inline holographic setup the sample is placed
closed to the pinhole which will restrict field of view (FOV) and diffraction signature of one particle (cell) will overlap w
ith other.
To avoid overlap of diffractio
n signatures and to increase FOV sample was placed close to CCD sensor. To test the working of
microscopy agarose microbeads were used. Optimization algorithm is used for reconstruction of object field from recorded
hologram.. Thus both amplitude as well a
s the phase images of the microbeads is reconstructed. Instead of using microscopic
objective to focus sample, autofocus algorithm is used to calculate the focused plane
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