Shaikh, S F
(2015)
Modelling of Magnetic Aggregation of Stem Cells in Microfluidics.
Masters thesis, Indian Institute of Technology Hyderabad.
Abstract
Individual cells assemble to form multi-cellular aggregates in a hierarchical manner over different length scales to coordinate the tissue functions. Current approaches to control the assembly and patterning of stem cells in 3D, require intrinsic adhesive properties, chemical modifications of cell and material interfaces or engineering intracellular interactions. A particular concern in surface modification of stem cell for adhesion properties, is the possibility of attenuation and inhibition of signalling pathways critical for differentiation of cells. Microfabrication technologies enable extremely fine-tuned culture management by means of control and reproducibility of extracellular stimulus (cues) to the levels unachievable by traditional standard tissue culture. Recent advances in microfabrication technologies and studies in microfluidics demonstrate the usage of magnetic forces that can be broadly applied across multiple length scales to direct individual cells for aggregation at microscales, and have long been used for large scale applications such as sorting and separating population of cells. Recent experiments suggest that magnetic microparticles (magMPs) can be efficiently incorporated in dose-dependent manner in extra-cellular environment of stem cells without any chemical and surface modifications of stem cells and assembly.
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