Bioengineering strategies for 3D bioprinting of tubular construct using tissue-specific decellularized extracellular matrix

Kamaraj, Meenakshi and Giri, Pravin Shankar and Mahapatra, Sandeep and Pati, Falguni and Rath, Subha Narayan (2022) Bioengineering strategies for 3D bioprinting of tubular construct using tissue-specific decellularized extracellular matrix. International Journal of Biological Macromolecules, 223. pp. 1405-1419. ISSN 0141-8130

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Abstract

The goal of the current study is to develop an extracellular matrix bioink that could mimic the biochemical components present in natural blood vessels. Here, we have used an innovative approach to recycle the discarded varicose vein for isolation of endothelial cells and decellularization of the same sample to formulate the decellularized extracellular matrix (dECM) bioink. The shift towards dECM bioink observed as varicose vein dECM provides the tissue-specific biochemical factors that will enhance the regeneration capability. Interestingly, the encapsulated umbilical cord mesenchymal stem cells expressed the markers of vascular smooth muscle cells because of the cues present in the vein dECM. Further, in vitro immunological investigation of dECM revealed a predominant M2 polarization which could further aid in tissue remodeling. A novel approach was used to fabricate vascular construct using 3D bioprinting without secondary support. The outcomes suggest that this could be a potential approach for patient- and tissue-specific blood vessel regeneration. © 2022

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IITH Creators:

IITH Creators	ORCiD
Pati, Falguni	http://orcid.org/0000-0002-3588-1800
Rath, Subha Narayan	UNSPECIFIED

Item Type:

Article

Additional Information:

The authors would like to acknowledge the Department of Biotechnology (DBT-BT/PR13381/MED/32/509/2015), Impacting Research Innovation and Technology (IMPRINT-2: IMP/2018/000785) and Ministry of Human Resource and Development (MHRD). Authors would like to acknowledge Mr. Viraj Mehta for assisting in EVOS microscope and Mr. Akshay Bhatt for cDNA preparation in vEC characterization.

Uncontrolled Keywords:

3D bioprinting; Tissue-specific dECM hydrogel; UMSC differentiation

Subjects:

Biomedical Engineering

Divisions:

Department of Biomedical Engineering

Depositing User:

Ms Palak Jain

Date Deposited:

22 May 2023 09:49

Last Modified:

22 May 2023 09:49