Highly fluorescent polyethylene glycol-ascorbic acid complex for imaging and antimicrobial therapeutics

Appidi, Tejaswini and Ravichandran, Gayathri and Mudigunda, Sushma V. and Thomas, Aswathi and Jogdand, Anil B. and Kishen, Saurabh and Subramaniyam, Kalaivanan and Emani, Naresh and Prabusankar, G and Rengan, Aravind Kumar (2021) Highly fluorescent polyethylene glycol-ascorbic acid complex for imaging and antimicrobial therapeutics. Materials Today Communications, 29. pp. 1-10. ISSN 2352-4928

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Abstract

In this report, a facile and eco-friendly method was developed to synthesize a potent antimicrobial agent, effective against fungus, bacteria, and virus. The biocompatible FDA approved polymer, polyethylene glycol (PEG), has been modified by ascorbic acid to form fluorescent polyethylene glycol-ascorbic acid complex (FL PEG AA). The flourescence of FL PEG AA complex could be tuned from green to red in the visible range with varying ascorbic acid(AA) concentrations. The Density Functional Theory (DFT) and the Time-Dependent Density Functional Theory (DT-DFT) on ascorbic acid–monoethylene glycol and ascorbic acid–hexaethylene glycol studies were performed to understand the electronic and bonding nature of FL PEG-ascorbic acid (FL PEG AA) system. The emission of FL PEG AA can be ascribed to the integrated core of FL PEG AA through strong hydrogen-bonding interactions. The fluorescence of FL PEG AA was successfully applied to understand its cellular uptake by breast cancer cells. The FL PEG AA complex showed significant inhibition of growth, when tested against fungus (Candida albicans), bacteria: gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) and the virus (Bacteriophage lambda). The antimicrobial activity of the FL PEG AA system supports the application of this novel formulation as a potent antimicrobial agent. Due to its viscous physical nature, the FL PEG AA complex could be applied as a coating agent or aerosol spray. © 2021 Elsevier Ltd

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IITH Creators:

IITH Creators	ORCiD
Emani, Naresh Kumar	https://orcid.org/0000-0002-0488-921X
Prabusankar, G	UNSPECIFIED
Rengan, Aravind Kumar	https://orcid.org/0000-0003-3994-6760

Item Type:

Article

Additional Information:

The authors thank the Department of Science & Technology (DST), the Department of Biotechnology (DBT), the Ministry of Human Resources & Development (MHRD), Government of India, for funding the following projects: DST-Inspire ( DST/INSPIRE/04/2015/000377 ), DBTNNT( BT/NNT/28/1386/2017 ), DST-AMT( DST/TDT/AMT/2017/227(G) ), & MHRD IMPRINT ( 4291 ). The authors also thank Vasudha Foundations , Hyderabad, for their generous financial support. The authors thank Dr. Manjula Reddy, Chief Scientist, CSIR -Centre for Cellular and Molecular Biology Hyderabad for providing the bacteriophage lambda ( ATCC 23724-B2 ) cultures. Authors A.T., G.R., and S.M., thank MHRD for providing a fellowship. Author T.A. thank the DST for an Inspire fellowship (IF160291).

Uncontrolled Keywords:

Antimicrobial properties; Bioimaging; DFT calculations; fluorescent polyethylene glycol (FL PEG); Polyethylene glycol (PEG)

Subjects:

Electrical Engineering
Biomedical Engineering
Chemistry

Divisions:

Department of Biomedical Engineering
Department of Chemistry
Department of Electrical Engineering

Depositing User:

. LibTrainee 2021

Date Deposited:

25 Aug 2022 13:51

Last Modified:

25 Aug 2022 13:51