Singh, Arvind Kumar and Singh, Shiv Govind
(2018)
Miniaturization of PCR Machine.
Masters thesis, Indian Institute of Technology Hyderabad.
Abstract
The polymerase chain reaction (PCR) is a technique defined for copying specific DNA sequences. The three basic steps in that process - splitting a DNA template into its two single strands (called denaturation); adding short segments of complementary DNA called primers to initiate replication of a chosen DNA sequence (annealing); and adding DNA polymerase to synthesis the complementary strand (called extension) - are repeated again and again to amplify the sequence. Each of these steps occurs optimally at a different temperature, so heating and cooling is carried out with an instrument called a thermal cycler. Better than the conventional big size PCR here we have designed a circuit which is capable of performing fast temperature rise and fall and that is within small region. This design supports the easy transportability of the machine because of its smaller size with very low design cost.
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