Hossain, Mehedi
(2016)
Study on protein-protein interaction between hR ad51 and
Alk B H 3.
Masters thesis, Indian Institute of Technology Hyderabad.
Abstract
In this study, the interaction between the human proteins ABH3 and HRAD51, which are the human homologs of the E.coli proteins AlkB and RecA respectively, is analysed. The proteins, which are His-tagged are purified using E.coli expression system. The interaction between the proteins is analyzed using FPLC system. Even though the bacterial proteins have been shown to interact, the same could not be shown for the human homologs in this study.
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