On-spot biosensing device for organophosphate pesticide residue detection in fruits and vegetables

Mukherjee, Subhankar and Pal, Souvik and Paria, Prasenjit and Bhattacharyya, Soumyadeb and Ghosh, Koustuv and Pal, Abhra and Ghosh, Devdulal and Singh, Om Krishan and et al, . (2021) On-spot biosensing device for organophosphate pesticide residue detection in fruits and vegetables. Current Research in Biotechnology, 3. pp. 308-316. ISSN 2590-2628

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Abstract

Acetylcholinesterase (AChE), a widely used enzyme for inhibition-based biosensors in pesticide residues detection, lags due to multiple-step operation, time-consuming incubation and reactivation/regeneration steps. Herein, this endeavour reports the development of Organophosphate Hydrolase (OPH), which has functional superiority over the AChE and explored in on-spot biosensing device for organophosphate pesticide residue detection in fruits and vegetables. The organophosphate degrading enzyme OPH is expressed from the ‘opd’ gene through biotechnological tools. The OPH exhibited its best activity at pH 8.0 and subsequently thermal inactivation over 37 °C. The activity of the purified OPH enzyme was found 2.75 U mL−1 at λmax 410 nm. Furthermore, the developed OPH is integrated into 96 well plate format with our previously reported UIISScan 1.1, an advanced imaging array technology based field-portable high-throughput sensory system. The developed biosensor revealed a linear range from 100 ng mL−1 to 0.1 ng mL−1 for detection of organophosphate pesticide residues with a negative slope i.e. y = 235.678x (ng mL−1) – 62.8725 with R2 = 0.99991 and n = 23. Moreover, the applicability of the developed biosensor was tested for market available fruits and vegetables. This is the first-ever reported OPH mediated on-spot biosensing device for pesticide residue detection in fruits and vegetables to the best of our knowledge. © 2021 The Author(s)

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IITH Creators:

IITH Creators	ORCiD

Item Type:

Article

Additional Information:

Authors acknowledge the funding by Electronics System Development and Application Division (ESDA), Ministry of Electronics and Information Technology (MeitY), Government of India [Grant ID: 26(4)/2019-ESDA, dated 23/01/2020] and ICAR-National Agricultural Science Fund (NASF) [Grant ID: NASF/MECH-8015/2019-20, dated: 29/02/2020]. The authors are also thankful to the Ministry of Electronics and Information Technology (MeitY), Government of India, for their continuous support.

Uncontrolled Keywords:

Biosensor; Enzyme; Fruits and vegetables; Imaging array; Organophosphate hydrolase; Pesticides

Subjects:

Electrical Engineering

Divisions:

Department of Electrical Engineering

Depositing User:

. LibTrainee 2021

Date Deposited:

12 Sep 2022 09:05

Last Modified:

12 Sep 2022 09:05