Mononuclear Co(ii) polypyridyl complexes: synthesis, molecular structure, DNA binding/cleavage, radical scavenging, docking studies and anticancer activities

Karumban, Kalai Selvan and Muley, Arabinda and Raut, Rajnikant and Gupta, Parth and Giri, Bishnubasu and Kumbhakar, Sadananda and Misra, Ashish and Maji, Somnath (2022) Mononuclear Co(ii) polypyridyl complexes: synthesis, molecular structure, DNA binding/cleavage, radical scavenging, docking studies and anticancer activities. Dalton Transactions, 51 (18). pp. 7084-7099. ISSN 1477-9226

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Abstract

Mononuclear Co(ii) complexes [CoII(L)Cl2]; 1, [CoII(L)(bpy)Cl]PF6; 2, [CoII(L)(phen)Cl]PF6; 3 and [CoII(L)(pic)Cl]; 4, (where L = N,N-bis(pyridin-2-ylmethyl)aniline, bpy = 2,2′-bipyridine, phen = 1,10-phenanthroline, pic = picolinic acid) were systematically synthesized and characterized by different analytical and spectroscopic methods. All the complexes were structurally identified by single-crystal X-ray diffraction analysis. Penta-coordinated complex 1 adopted a distorted trigonal bipyramidal geometry, whereas hexacoordinated complexes 2-4 have distorted octahedral geometry. The interactions of salmon sperm DNA (ss-DNA) with our synthesized complexes 1-4 were investigated by absorbance and fluorescence spectroscopy. All the complexes are very susceptible to DNA binding and exhibited binding affinities (Kb) in the order of ∼104 M−1, indicating their strong interaction with ss-DNA. The Stern-Volmer constant (Ksv) ranged from 0.46 ± 0.01 × 104 to 1.08 ± 0.04 × 104 M−1, suggesting weak or moderate binding with DNA. Agarose gel electrophoresis revealed the DNA cleavage activity in vitro for 2-4, which could efficiently cleave the supercoiled plasmid DNA without any external agents; however, with the addition of H2O2, the cleavage property was enhanced. Live-cell imaging and other biochemical assays demonstrated the ability of Co(ii) complexes 1-4 to induce significant cytotoxicity in A549 lung cancer cells with IC50 values of 32.14 ± 1.3 μM, 3.14 ± 0.16 μM, 15.78 ± 0.72 μM and 18.45 ± 0.92 μM, and in MDA-MB-231 breast cancer cells with IC50 values of 20.42 ± 0.92 μM, 0.41 ± 0.02 μM, 2.31 ± 0.12 μM and 9.67 ± 0.35 μM, respectively. © 2022 The Royal Society of Chemistry.

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IITH Creators:

IITH Creators	ORCiD
Misra, Ashish	https://orcid.org/0000-0002-3042-8302
Maji, Somnath	http://orcid.org/0000-0001-6047-1339

Item Type:

Article

Additional Information:

Financial support received from the Council of Scientific and Industrial Research (CSIR) (fellowship to K. S., A. M., R. R. and B. G.), Science and Engineering Research Board (SERB) (fellowship to S. K.), Department of Science and Technology, India: project no. ECR/2016/000382; and Indian Institute of Technology Hyderabad for the instrumentations are gratefully acknowledged. P. G. acknowledges fellowship support from the Ministry of Education. A. M. acknowledges funding support from DST SERB (ECR/2017/002544) and IIT Hyderabad.

Uncontrolled Keywords:

Analytical method; Anticancer activities; Bipyridines; Complex 1; DNA-binding; Docking studies; Phenanthrolines; Picolinic acid; Radical scavenging; Synthesised

Subjects:

Others > Biotechnology
Chemistry

Divisions:

Department of Biotechnology
Department of Chemistry

Depositing User:

. LibTrainee 2021

Date Deposited:

21 Jul 2022 06:16

Last Modified:

21 Jul 2022 06:16

URI:

http://raiithold.iith.ac.in/id/eprint/9833