Galactose Tethered Decellularized Liver Matrix: Toward a Biomimetic and Biofunctional Matrix for Liver Tissue Engineering

Sasikumar, Shyama and Boden, Andrew and Pati, Falguni and et al, . (2022) Galactose Tethered Decellularized Liver Matrix: Toward a Biomimetic and Biofunctional Matrix for Liver Tissue Engineering. ACS Applied Bio Materials, 5 (6). pp. 3023-3037. ISSN 2576-6422

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Abstract

The major challenge in liver tissue engineering is the replication of the microenvironment and microarchitecture of the liver tissue at the nanoscale. Decellularized liver matrix (DLM) provides an ideal material for scaffold preparation, as it retains the relevant structural and biochemical composition. However, the loss of bioactive factors during decellularization needs to be taken into account when using DLM and should be supplemented accordingly for an expected outcome. This study reports on the modification of DLM by the addition of galactose residues using a two-step thiol-ene-mediated photoclick chemistry for the coupling of galactose moieties to the DLM. Modification with galactose enhanced the function of hepatocytes and provides many advantages over currently used DLM and DLM-based materials. The galactose modified DLM enhanced the initial HepG2 cell adhesion to the substrate with changes in dynamics over time such as spheroid formation and further migration on the matrix. Our observation is that the galactose ligand decoration can also enhance the liver-specific metabolism of HepG2 compared to unmodified DLM. Galactosylated DLM also showed a better establishment of cellular polarity which also contributes to the function of HepG2 cells. Together our results demonstrate the advantages of adding galactose residues to currently available biomaterials, which makes this approach an attractive method for ECM-based liver tissue engineering. ©

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IITH Creators:

IITH Creators	ORCiD
Pati, Falguni	http://orcid.org/0000-0002-3588-1800

Item Type:

Article

Additional Information:

The authors would like to acknowledge Ramalingaswami Research Fellowship from the Department of Biotechnology, Government of India (BT/HRD/35/02/2006). In addition, S.S. would like to thank the Department of Biotechnology, Government of India for the DBT-JRF fellowship and the Swinburne University of Technology, Melbourne, Australia, for funding the Swinburne University Postgraduate Research Award (SUPRA).

Uncontrolled Keywords:

biomaterials; galactosylation; liver extracellular matrix; liver tissue engineering; substrate modification

Subjects:

Others > Biological sciences
Others > Biotechnology
Biomedical Engineering
Chemistry
Materials Engineering > Materials engineering

Divisions:

Department of Biomedical Engineering
Department of Biotechnology
Department of Chemistry
Department of Material Science Engineering

Depositing User:

. LibTrainee 2021

Date Deposited:

16 Jul 2022 06:43

Last Modified:

16 Jul 2022 06:43