New insights into inhibitory nature of triclosan on acetylcholinesterase activity

Pullaguri, Narasimha and Kagoo, Andrea R and Bhargava, Anamika (2022) New insights into inhibitory nature of triclosan on acetylcholinesterase activity. Toxicology, 466. ISSN 0300_483X

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Abstract

The antimicrobial agent, triclosan, has been designated as a “contaminant of emerging concern (CEC)”. Previous in vivo studies have shown that triclosan exposure can inhibit acetylcholinesterase (AChE) activity. However, mechanistic insights into AChE inhibition by triclosan are missing. Here, using in vitro activity assay with purified AChE, we show that triclosan can directly inhibit AChE. In vivo, triclosan exposure resulted in reduced total antioxidant capacity concomitant with reduced AChE activity in the adult zebrafish brain. Adult zebrafish when pre-treated with antioxidant melatonin, resulted in attenuated oxidative stress and attenuated inhibitory effect of triclosan on the AChE activity. Our results indicate that triclosan can affect AChE activity both by direct binding and indirectly through increased oxidative stress and therefore, provide important mechanistic insights into triclosan-induced neurotoxicity. © 2021 Elsevier B.V.

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IITH Creators:

IITH Creators	ORCiD
Bhargava, Anamika	http://orcid.org/0000-0002-1048-3427

Item Type:

Article

Additional Information:

This work was supported by the Indian Institute of Technology Hyderabad (IITH) and ECR-SERB-DST ( ECR/2017/000242 ) grants to AB and Ministry of Education, India fellowship to NP and AK. The authors thank Dr Yogesh Bhargava (Dr HS Gour central university, Sagar, M.P, India) for his critical comments on the manuscript and help with the graphical abstract.

Uncontrolled Keywords:

Acetylcholinesterase; In vitro; Melatonin; Oxidative stress; Triclosan; Zebrafish

Subjects:

Others > Biotechnology

Divisions:

Department of Biotechnology

Depositing User:

. LibTrainee 2021

Date Deposited:

14 Jul 2022 09:32

Last Modified:

14 Jul 2022 09:32