Biocompatibility-on-a-chip: Characterization and evaluation of decellularized tendon extracellular matrix (tdECM) hydrogel for 3D stem cell culture in a microfluidic device

Bhatt, Akshay and Pati, Falguni and Rath, Subha Narayan and et al, . (2022) Biocompatibility-on-a-chip: Characterization and evaluation of decellularized tendon extracellular matrix (tdECM) hydrogel for 3D stem cell culture in a microfluidic device. International Journal of Biological Macromolecules, 213. pp. 768-779. ISSN 0141-8130

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Abstract

Researchers have always tried expensive in vitro tests to show the 3D usability of dECM. The use of tissue-specific hydrogels in a microfluidic device is rarely studied. In this study, we have used ECM obtained from goat digital flexor tendons by decellularization technique. The tdECM was characterized for its structural properties using Scanning Electron Microscopy (SEM). Collagen, dsDNA, GAGs, and protein contents were quantified using spectrophotometric assays. The cell viability and proliferation of human umbilical cord-derived mesenchymal stem cells (hUMSCs) encapsulated in the tdECM hydrogel inside the microfluidic device were checked using Calcein-AM/PI. The FTIR data showed prominent peaks of the amide group, indicating the presence of collagen. The SEM data showed intact fiber morphology after the decellularization process. There was a 95 % reduction in double-stranded DNA (dsDNA) content, proving the effectiveness of the decellularization technique. There was no significant difference in the collagen content of tdECM and the GAGs were also in the acceptable range compared to the native tissue. Over 90 % cell viability in hUMSCs was observed qualitatively and quantitatively in vitro and inside a microfluidic device. In conclusion, we characterized the tdECM hydrogel and demonstrated its compatibility with the microfluidic device. © 2022 Elsevier B.V.

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IITH Creators:

IITH Creators	ORCiD
Pati, Falguni	http://orcid.org/0000-0002-3588-1800
Rath, Subha Narayan	https://orcid.org/0000-0002-3414-2328

Item Type:

Article

Additional Information:

The Department of Science and Technology funded the work, Government of India, Nano mission project SR/NM/NT/-1095/2016 , ICMR 35/5/2019-NANO/BMS , ICMR IMP/2018/000785 , DST-Nidhi Prayas seed grant, and MHRD government of India. The authors would like to acknowledge the DST-FIST (SR/FST/ETI-421/2016) for the FESEM characterization. The authors would like to acknowledge Ms. Amruta Datla for her assistance with the SDS-PAGE.

Uncontrolled Keywords:

Decellularization; Microfluidic model; Tendon-derived extracellular matrix (tdECM) hydrogel; Umbilical cord-derived mesenchymal stem cells (hUMSCs)

Subjects:

Biomedical Engineering

Divisions:

Department of Biomedical Engineering

Depositing User:

. LibTrainee 2021

Date Deposited:

27 Jul 2022 05:19

Last Modified:

27 Jul 2022 05:19