Biomimicking nanofibrous gelatin microspheres recreating the stem cell niche for their ex-vivo expansion and in-vivo like differentiation for injectable stem cell transplantation

Sarviya, Nandini and Basu, Suparna Mercy and Giri, Jyotsnendu and et al, . (2022) Biomimicking nanofibrous gelatin microspheres recreating the stem cell niche for their ex-vivo expansion and in-vivo like differentiation for injectable stem cell transplantation. Biomaterials Advances, 139. pp. 1-17. ISSN 2772-9508

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Abstract

Stem cells based novel treatment modality for degenerative and immune dysfunction diseases created a huge demand of suitable carriers to support ex-vivo production of quality stem cells, and effective in-vivo transplantation of stem cells and their fate. In spite of promising candidature of nanofibrous microspheres (NFM) to recreate native stem cell niches to be used for possible scaling-up for ex-vivo stem cells expansion, it remains fairly unexplored. A systematic study on the stem cell-NFM interaction comparative with commercial microspheres (CM) has been performed for the first time. Gelatin NFM with variable physicochemical properties such as size, surface properties, surface chemistry, and variable degradability were prepared using microemulsion coupled with thermally induced phase separation (TIPS) method. Effect of physicochemical properties of NFM and their cellular interaction such as binding, morphology, metabolic activity and proliferation studies were performed using human bone marrow-derived mesenchymal stem cells (hBMSCs), human dental follicle stem cells (hDFSCs) and human gingival fibroblast (HGF) cells and compared with the commercial and solid microspheres. Gelatin NFM supports excellent cell binding, proliferation, metabolic activities and chemical cues specific differentiation. All out-turns indicate that NFM stand to be an outstanding candidate for ex-vivo cells' expansion and injectable carriers for stem cell transplantation. © 2022 Elsevier B.V.

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IITH Creators:

IITH Creators	ORCiD
Giri, Jyotsnendu	UNSPECIFIED

Item Type:

Article

Additional Information:

This work was financially supported by the Department of Biotechnology, Ministry of Science and Technology, India ( BT/RLF Re-entry/2013 ) and Department of Science and Technology, Ministry of Science and Technology, Science and Engineering Research Board, India for Project nos- SB/S3/CE/048/2015 and CRG/2020/005244 .

Uncontrolled Keywords:

Biocompatible; Gelatin; Injectable cell carrier; Nanofibrous microsphere; Tissue regeneration

Subjects:

Others > Biotechnology
Biomedical Engineering
Chemistry

Divisions:

Department of Biomedical Engineering
Department of Biotechnology
Department of Chemistry

Depositing User:

. LibTrainee 2021

Date Deposited:

27 Jul 2022 05:20

Last Modified:

27 Jul 2022 05:20