Gamma-Linolenic Acid (GLA) Protects against Ionizing Radiation-Induced Damage: An In Vitro and In Vivo Study

Rengachar, P. and Bhatt, A.N. and Das, Undurti N and et al, . (2022) Gamma-Linolenic Acid (GLA) Protects against Ionizing Radiation-Induced Damage: An In Vitro and In Vivo Study. Biomolecules, 12 (6). pp. 1-36. ISSN 2218-273X

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Abstract

Radiation is pro-inflammatory in nature in view of its ability to induce the generation of reactive oxygen species (ROS), cytokines, chemokines, and growth factors with associated inflammatory cells. Cells are efficient in repairing radiation-induced DNA damage; however, exactly how this happens is not clear. In the present study, GLA reduced DNA damage (as evidenced by micronuclei formation) and enhanced metabolic viability, which led to an increase in the number of surviving RAW 264.7 cells in vitro by reducing ROS generation, and restoring the activities of desaturases, COX-1, COX-2, and 5-LOX enzymes, TNF-Î±/TGF-Î², NF-kB/IkB, and Bcl-2/Bax ratios, and iNOS, AIM-2, and caspases 1 and 3, to near normal. These in vitro beneficial actions were confirmed by in vivo studies, which revealed that the survival of female C57BL/6J mice exposed to lethal radiation (survival~20%) is significantly enhanced (to ~80%) by GLA treatment by restoring altered levels of duodenal HMGB1, IL-6, TNF-Î±, and IL-10 concentrations, as well as the expression of NF-kB, IkB, Bcl-2, Bax, delta-6-desaturase, COX-2, and 5-LOX genes, and pro-and anti-oxidant enzymes (SOD, catalase, glutathione), to near normal. These in vitro and in vivo studies suggest that GLA protects cells/tissues from lethal doses of radiation by producing appropriate changes in inflammation and its resolution in a timely fashion. Â© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

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IITH Creators:

IITH Creators	ORCiD

Item Type:

Article

Additional Information:

This work was supported by a grant from INMAS/DRDO (project sanction letter no. TC12519/INM-03/CARS/2011 dated: 22 December 2011), India and Department of Atomic Energy (DAE) (project sanction letter no. 35/14/38/2016-BRNS-35173), India to UND.

Uncontrolled Keywords:

cytokines, inflammation, lipoxin A4, prostaglandins, radiation, survival

Subjects:

Biomedical Engineering

Divisions:

Department of Biotechnology

Depositing User:

. LibTrainee 2021

Date Deposited:

23 Jun 2022 09:00

Last Modified:

23 Jun 2022 09:00