Mononuclear cobalt(II) complexes with polypyridyl ligands: Synthesis, characterization, DNA interactions and in vitro cytotoxicity towards human cancer cells

Karumban, K.S. and Misra, Ashish and Maji, Somnath and et al, . (2022) Mononuclear cobalt(II) complexes with polypyridyl ligands: Synthesis, characterization, DNA interactions and in vitro cytotoxicity towards human cancer cells. Journal of Inorganic Biochemistry, 233. pp. 1-15. ISSN 0162-0134

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Abstract

Mononuclear cobalt(II) complexes [CoII(L1)Cl2]; 1, [CoII(L1)(bpy)Cl]PF6; 2, [CoII(L1)(phen)Cl]PF6; 3 and [CoII(L2)Cl2]; 4 (where L1 = N,N-bis(pyridin-2-ylmethyl)aniline, L2 = (2,4,6-trimethyl-N,N-bis(pyridin-2-ylmethyl)aniline, bpy = 2,2/−bipyridine, phen = 1,10-phenanthroline) were synthesized and characterized by different analytical and spectroscopic methods. All the complexes were structurally identified by single-crystal X-ray crystallography. Penta-coordinated complex 1 adopted distorted trigonal bipyramidal and hexacoordinated complexes 2 and 3 having distorted octahedral geometry whereas tetra-coordinated complex 4 has distorted tetrahedral geometry. The interactions of salmon sperm DNA (ss-DNA) with complexes (1-4) were investigated by absorbance, fluorescence spectroscopy and molecular docking studies. All the complexes are very susceptible to DNA binding and the binding affinity (Kb) follows the order 3 (2.05 × 104 M −1) > 4 (1.40 × 104 M −1) > 2 (1.36 × 104 M −1) > 1 (1.34 × 104 M −1) indicating they have superior DNA binding ability. The Stern-Volmer constant (Ksv) ranges from 1.10 × 104 M −1 to 1.95 × 104 M −1 suggesting weak or moderate binding with DNA. DNA cleavage study in plasmid DNA reveals very efficient DNA cleavage factors even in the absence of any external agents. Using multiple biochemical assays, we have demonstrated that 1–4 induces apoptosis of human cancer cells with IC50 values of 26.48 ± 1.45 μM, 10.89 ± 0.55 μM, 7.63 ± 0.4 μM and 37.67 ± 2.06 μM, respectively in A549 lung adenocarcinoma cells and 14.45 ± 0.73 μM, 1.97 ± 0.1 μM, 0.98 ± 0.05 μM and 24.43 ± 1.22 μM, respectively in MDA-MB-231 breast adenocarcinoma cells. © 2022

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IITH Creators:

IITH Creators	ORCiD
Misra, Ashish	https://orcid.org/0000-0002-3042-8302
Maji, Somnath	http://orcid.org/0000-0001-6047-1339

Item Type:

Article

Additional Information:

Financial support received from the Council of Scientific and In- dustrial Research (CSIR) (fellowship to K.S., R.R., A.M., and B.G.), Sci- ence and Engineering Research Board (SERB) (fellowship to S.K.), Department of Science and Technology, India: project no. ECR/2016/ 000382; and Indian Institute of Technology Hyderabad for the instrumentations are gratefully acknowledged. P.G. acknowledges fellowship support from the Ministry of Education. A.M. acknowledges funding support from DST SERB (ECR/2017/002544) and IIT Hyderabad.

Uncontrolled Keywords:

Cobalt(II) complexes, Crystal structure, Cytotoxicity, DNA binding/cleavage, Molecular docking, Polypyridyl ligands

Subjects:

Others > Biotechnology
Chemistry

Divisions:

Department of Biotechnology
Department of Chemistry

Depositing User:

. LibTrainee 2021

Date Deposited:

21 Jun 2022 04:26

Last Modified:

22 Jun 2022 08:49