Towards point-of-care diagnosis of Alzheimer’s disease: Multi-analyte based portable chemiresistive platform for simultaneous detection of β-amyloid (1–40) and (1–42) in plasma

Supraja, P. and Tripathy, S. and Singh, R. and Singh, V. and Chaudhury, G. and Singh, S.G. (2021) Towards point-of-care diagnosis of Alzheimer’s disease: Multi-analyte based portable chemiresistive platform for simultaneous detection of β-amyloid (1–40) and (1–42) in plasma. Biosensors and Bioelectronics, 186 (113294). ISSN 09565663 (Submitted)

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Abstract

Label-free simultaneous detection of Alzheimer's disease (AD) specific biomarkers Aβ40 and Aβ42 peptides on a single platform using polypyrrole nanoparticle-based chemiresistive biosensors is reported here. The proposed interdigitated-microelectrode based inexpensive multisensor-platform can concurrently detect Aβ40 and Aβ42 in spiked-plasma in the range of 10-14 – 10-6 g/mL (with LoDs being 5.71 and 9.09 fg/mL, respectively), enabling the estimation of diagnostically significant Aβ42/Aβ40 ratio. A detailed study has been undertaken here to record the individual sensor responses against spiked-plasma samples with varying amounts and proportions of the two target peptides, towards enabling disease-progression monitoring using the Aβ-ratio. As compared to the existing cost-ineffective brain-imaging techniques such as PET and MRI, and the high-risk CSF based invasive AD biomarkers detecting procedures, the proposed approach offers a viable alternative for affordable point-of-care AD diagnostics, with possible usage in performance evaluation of therapeutic drugs. Towards point-of-care applications, the portable readout used in this work was conjugated with an android-based mobile app for data-acquisition and analysis. © 2021 Elsevier B.V.

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IITH Creators:
IITH CreatorsORCiD
Singh, Shiv Govindhttp://orcid.org/0000-0001-7319-879X
Item Type: Article
Uncontrolled Keywords: Electronic readout, Mobile interfacing, Point-of-care, Polypyrrole-chemiresistive, Portable sensors, Alzheimer's
Subjects: Electrical Engineering
Divisions: Department of Electrical Engineering
Depositing User: Mrs Haseena VKKM
Date Deposited: 20 Apr 2022 05:37
Last Modified: 20 Apr 2022 05:37
URI: http://raiithold.iith.ac.in/id/eprint/9218
Publisher URL: https://www.sciencedirect.com/science/article/pii/...
OA policy: https://v2.sherpa.ac.uk/id/publication/15498
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