A simple monophasic LGPY medium for routine maintenance of Leishmania donovani promastigotes

Abhishek, S and Chaturvedi, S and Gupta, A and Ahmed, Ghufran and Das, Vidyanand Ravi and Narayan, Shyam (2021) A simple monophasic LGPY medium for routine maintenance of Leishmania donovani promastigotes. Parasitology Research, 120 (8). pp. 2969-2971. ISSN 09320113

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Abstract

Here, we report a simple, economic and autoclavable monophasic LGPY medium supplemented with 10% fetal bovine serum (FBS), for routine maintenance of Leishmania donovani promastigotes for laboratory use. In comparison to commercially available M199 and RPMI-1640 media, LGPY has shown approximately seven fold more cell growth. The parasite has been observed to survive in the medium for at least 15 days post-inoculation. The medium also supports long-term sub-passaging of the promastigotes and can also be stored at 4 °C or room temperature for 14 months and 45 days, respectively.

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IITH Creators:	IITH Creators ORCiD
Item Type:	Article
Uncontrolled Keywords:	Culture medium, Growth kinetics, Leishmania, Monophasic medium
Subjects:	Others > Biotechnology
Divisions:	Department of Biotechnology
Depositing User:	Mrs Haseena VKKM
Date Deposited:	02 Nov 2021 10:53
Last Modified:	02 Nov 2021 10:53
URI:	http://raiithold.iith.ac.in/id/eprint/8884
Publisher URL:	https://link.springer.com/10.1007/s00436-021-07193...
OA policy:	https://v2.sherpa.ac.uk/id/publication/8149
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