Sequential optimization and large scale production of lipase using tri-substrate mixture from Aspergillus niger MTCC 872 by solid state fermentation

Mandari, Venkatesh and Nema, Ashutosh and Devarai, Santhosh Kumar (2020) Sequential optimization and large scale production of lipase using tri-substrate mixture from Aspergillus niger MTCC 872 by solid state fermentation. Process Biochemistry, 89. pp. 46-54. ISSN 1359-5113

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Abstract

Tri-substrate mixture of Prosopis juliflora (PJ), red gram husk (RGH) and cotton seed cake (CSC) has been studied for the production of lipase (E.C. 3.1.1.3) using Aspergillus niger MTCC 872 in solid state fermentation. Simplex centroid mixture design (SCMD) was implemented to optimize the tri-substrate mixture composition consisting of PJ, RGH and CSC. Mixture taken in the ratio of 6.66:1.66:1.66 for PJ:RGH:CSC has shown highest lipase activity of 212.20 ± 6.36 U/gds at 30 °C, 7 pH and 70% initial moisture content (v/w). Sequential optimization of physical parameters was done using the central composite face-centered design (CCFD). The optimum mixture composition has shown the highest lipase activity of 269.87 ± 8.09 U/gds at 35 °C, 7 pH and 70% initial moisture content (v/w). ANOVA analysis for SCMD and CCFD confirms the model’s significance with R2 values of 0.9989 and 0.968. A 1.27 fold increased lipase activity was obtained after physical parameters optimization. Large scale production using 1 kg substrate was carried out in tray bioreactor with different bed heights and the highest lipase activity of 208.79 ± 6.26 U/gds was obtained. This study signifies the enhancement of lipase production using substrate PJ for lipase production along with the other agricultural residues.

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IITH Creators:

IITH Creators	ORCiD
Devarai, Santhosh Kumar	UNSPECIFIED

Item Type:

Article

Additional Information:

The authors are indebted to Dr. Kruthi Doriya and Mr. Anup Ashok for helping to conduct the experiments at the initial stages and their support whenever required in the laboratory. Special thanks to Ms. Swarnalatha for facilitating analysis of the elemental composition of the solid substrates. The authors are grateful to Science and Engineering Research Board - Department of Science and Technology , Government of India for supporting the research with grant number SERB-DST- ECR/2017/000241 .

Uncontrolled Keywords:

Central Composite Face-Centered Design (CCFD); Lipase; Prosopis Juliflora (PJ); Simplex Centroid Mixture Design (SCMD); Solid state fermentation (SSF); Specific activity

Subjects:

Chemical Engineering

Divisions:

Department of Chemical Engineering

Depositing User:

Team Library

Date Deposited:

11 Nov 2019 04:33

Last Modified:

18 Nov 2022 11:08

URI:

http://raiithold.iith.ac.in/id/eprint/6934