Jose, Jiya and Anas, Abdulaziz and Ch, Subrahmanyam and et al, .
(2019)
The extinction of antimicrobial resistant pathogens using silver embedded silica nanoparticles and an efflux pump blocker.
ACS Applied Bio Materials.
ISSN 2576-6422
(In Press)
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Abstract
Control measures against antimicrobial resistant bacterial pathogens are important challenges in our daily life. In this study we discuss the sensitivity and resistance of four bacterial pathogens, Vibrio alginolyticus, Escherichia coli, Staphylococcus aureus and Bacillus subtilis, to silica nanoparticles embedded with silver nanoparticles. Successively, by combining with an efflux pump blocking agent Verapamil, we find that the silver-silica nanoparticle induces complete mortality to even the most resistive S. aureus. The above pathogens are selected from a pool of 100 bacterial strains resistant to silver nitrate. While S. aureus shows increased resistance to the nanoparticles, the cell wall integrity and genetic stability of V. alginolyticus and E. coli are lost in the presence of silver-silica nanoparticles. This study suggests that the antimicrobial properties of the nanoparticles against Gram negative pathogens originates from an increased oxidative stress, which is confirmed by blocking of reactive oxygen species using scavengers such as ascorbic acid and observing DNA damage. The antimicrobial property of the nanoparticle when combined with its nontoxic nature to mammalian cells makes it a promising agent for controlling drug-resistant Gram negative pathogens.
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