Single Neuron Imaging Reveals Metabotropic Glutamate Receptor-Mediated Bursting and Delay in Calcium Oscillation in Hippocampal Neurons

Singh, Ranjana and Saxena, Abha and Giri, Lopamudra (2019) Single Neuron Imaging Reveals Metabotropic Glutamate Receptor-Mediated Bursting and Delay in Calcium Oscillation in Hippocampal Neurons. In: 41 st Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBS), 23-27 July 2019, Berlin, Germany.

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Abstract

Measurement of cytosolic calcium activity in single neuron and quantification of response to drug treatment over a longer period remain challenging. Especially, analysis of amplitude, frequency, and delay in calcium response in G-protein coupled receptors (GPCR) targeting drug-treated neurons is complicated due to inherent heterogeneity. To address this, we have utilized k- means clustering to identify various sub-populations. Here we focus on measuring drug dose response in the single neuron by means of high-resolution confocal imaging. Metabotropic glutamate receptor was activated using 3,5-dihydroxyphenylglycine (DHPG). The result reveals an unusual delay in calcium response for higher dose particularly in responding neurons. The proposed methodology can be used for selection of drug dose and optimization of the time window for neurodegenerative diseases.

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IITH Creators:	IITH Creators ORCiD Giri, Lopamudra http://orcid.org/0000-0002-2352-7919
Item Type:	Conference or Workshop Item (Paper)
Uncontrolled Keywords:	Drugs , Neurons , Calcium , Imaging , Delays , Fluorescence , Transient analysis
Subjects:	Chemical Engineering
Divisions:	Department of Chemical Engineering
Depositing User:	Library Staff
Date Deposited:	16 Oct 2019 08:50
Last Modified:	16 Oct 2019 08:50
URI:	http://raiithold.iith.ac.in/id/eprint/6556
Publisher URL:	http://doi.org/10.1109/EMBC.2019.8856638
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