Promoted Osteoconduction of Polyurethane-Urea Based 3D Nanohybrid Scaffold through Nanohydroxyapatite Adorned Hierarchical Titanium Phosphate

Ghorai, Sanjoy Kumar and Maji, Somnath and Subramanian, Bhuvaneshwaran and et al, . (2019) Promoted Osteoconduction of Polyurethane-Urea Based 3D Nanohybrid Scaffold through Nanohydroxyapatite Adorned Hierarchical Titanium Phosphate. ACS Applied Bio Materials. ISSN 2576-6422

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Abstract

Lack of optimal physiological properties, bacterial colonization, and auto osteoinduction, are the foremost anxieties of orthopedic implantations. In terms of bone healing, many researchers have reported the release of additional growth factors of the implanted biomaterials to accelerate the bone regeneration process. However, the additional growth factor may cause side effects such as contagion, nerve pain, and the formation of ectopic bone. Thus, the design of osteoconductive scaffold having excellent biocompatibility, appropriate physico-mechanical properties, and promoted auto osteo-inductivity with antibacterial activity is greatly desired. In this study, 2D rod-like nanohydroxyapatite (nHA) adorned titanium phosphate (TP) with a flower-like morphology was synthesized by a hydrothermal precipitation reaction. The nanohybrid material (nHA-TP) was incorporated into the synthesized polycaprolactone diol and spermine based thermoplastic polyurethane-urea (PUU) via in situ technique followed by salt leaching to fabricate the macro-porous 3D polymer nanohybrid scaffold (PUU/nHA-TP). Structure explication of PUU was performed by NMR spectroscopy. The synthesized nanohybrid scaffold with 1% nHA-TP showed 67 % increase of tensile strength and 18% improved modulus compare to the pristine PUU via formation of H-bonding or dative bond between the metal and the amide linkage of the polyurethane or polyurea. In vitro study showing the improved cell viability and proliferation of the seeded cell, revealed the superior osteoconductivity of the nanohybrid scaffold. Most importantly, the in vivo experiments revealed a significant amount of bone regeneration in the nanohybrid scaffold implanted tibial site compare to the pristine scaffold without any toxic effect. Introduction of the minute amount of titanium phosphate within the adorned nHA promotes the osteoconductivity significantly by the capability of forming co-ordinate bond of the titanium ion. Depending upon the mechanical, physicochemical, in vitro characteristic and in vivo osteoconductivity, PUU/nHA-TP nanohybrid scaffold has great potentiality as an alternative biomaterial in bone tissue regeneration application.

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