COST EFFECTIVE PROCESS FOR PRODUCTION OF L- ASPARAGINASE BY SOLID STATE FERMENTATION AND SUBMERGED FERMENTATION USING Aspergillus terreus MTCC 1782

Lendekar, Vaibhav Krishna and Devarai, Santhosh Kumar (2019) COST EFFECTIVE PROCESS FOR PRODUCTION OF L- ASPARAGINASE BY SOLID STATE FERMENTATION AND SUBMERGED FERMENTATION USING Aspergillus terreus MTCC 1782. Masters thesis, Indian institute of technology Hyderabad.

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Abstract

L-Asparaginase plays important role in medical application, particularly in treatment of Acute Lymphoblastic Leukemia (ALL) as an anti-cancer compound. It also helps in removing acrylamide content found in starchy foods that is carcinogenic in nature. 5 The present work deals with comparative study to the production of extracellular L- Asparaginase from Aspergillus terreus MTCC 1782 using submerged fermentation (SmF) and Solid State Fermentation (SSF) Process. Study involves two new fermentation processes i.e Submerged fermentation using alginate beads containing cells and fermentation process with agriculture waste extraction of solid substrate using alginate beads with induced cells. Optimization of parameters was performed in the two fermentation process. In case of submerged fermentation, Highest L-Asparaginase activity of 12.68 U/ml was observed at 72 h, with optimized fermentation parameters were found to be at pH 6, inoculum volume of 4x10 6 cells, 180 rpm incubator speed at 30°C. Whereas, L-Asparaginase activity reached its maximum value of 21.37 U/ml within 48 h using immobilized cells with optimized initial cells of 4x10 6 cells entrapped in 3% of alginate bead at180 rpm at 30 °C. Potato peels has given the highest activity of 14.6 U/g of dry substrate in SSF among different substrates. Fermentation process with liquid extract of wheat bran has the ability to give maximum enzyme activity 11.05 U/ml, obtained using 4x10 6 cells per ml entrapped in 3% of alginate bead, 180 rpm at 30 °C. Pre-economic analysis was done for all fermentation processes, which gives outline about cost effective production of the enzyme.

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