Flexible ITO Electrode With Gold Nanostructures for Femtomolar DNA Hybridization Detection

Tripathy, Suryasnata and Joseph, Jose and Vanjari, Siva Rama Krishna and Rao, AVSS Narayana and Singh, Shiv Govind (2018) Flexible ITO Electrode With Gold Nanostructures for Femtomolar DNA Hybridization Detection. IEEE Sensors Letters, 2 (4). pp. 1-4. ISSN 2475-1472

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Abstract

In this article, we report detection of DNA hybridization using a gold nanostructure coated flexible indium tin oxide (ITO) working electrode, aiming at exploring alternate materials for developing low-cost light-weight sensing platforms in the future. Herein, gold nanostructures were electrodeposited onto the flexible ITO substrate with HAuCl 4 precursor solution. To prepare the bioelectrode for DNA sensing, thiol-modified single-stranded DNA probes specific to the BRCA1 gene were attached to the nanostructured gold surface via self-assembly. When subjected to hybridization detection in a wide concentration range of 1 fM-1 μM, the bioelectrode resulted in a limiting detection of 0.255 fM (calculated with 3.3σ method), with a sensitivity of 10.94 (μA/mol)/cm 2 . In order to estimate the efficiency of the sensor, we have performed interference analysis in the presence of a noncomplementary target DNA and human serum albumin. Furthermore, the reproducibility of the biosensor was verified using a blind testing protocol.

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