Single step grown MoS2 on pencil graphite as an electrochemical sensor for guanine and adenine: A novel and low cost electrode for DNA studies

Vishnu, Nandimalla and Badhulika, Sushmee (2019) Single step grown MoS2 on pencil graphite as an electrochemical sensor for guanine and adenine: A novel and low cost electrode for DNA studies. Biosensors and Bioelectronics, 124-12. pp. 122-128. ISSN 0956-5663

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Abstract

Herein we report a simple, one-step approach to prepare a low-cost and binder free MoS2-pencil graphite electrode (i.e., MoS2-PGE) for the electrochemical oxidation of DNA nucleobases i.e., guanine (G) and adenine (A) in physiological pH (7.4) buffer solution. MoS2-PGE was synthesised by hydrothermal method and the morphology of such hybrid was characterized by field emission scanning electron microscopy, X-ray diffraction, Raman and X-ray photoelectron spectroscopy. In cyclic voltammetry, MoS2-PGE displays two well-seprated and well-defined irresversible peaks at 0.58 and 0.90 V for electrochemical oxidation of G and A respectively when compared to bare PGE. Likewise, differential pulse voltammetry of MoS2-PGE showed well-seprated and sharp peak current responses for G and A at 0.56 V and 0.85 V respectively. Under optimized conditions, DPV was further adopted for simultaneous and separation-free determination of G and A in physiological pH. MoS2-PGE shows good stability with linear range of 15–120 μM and 15–120 μM for G and A detection respectively. Obtained sensitivity and limit of detection (signal-to-noise = 3) are comparable with the previous literature. As an immediate practical applicability, MoS2-PGE was used for quantification of G and A concentration in calf-thymus DNA and detected ratio of G and A (i.e., [G]/[A]) ratio is 0.85. The current approach provides a new avenue towards the development of affordable electrodes for a wide range of bioanalytical applications

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IITH Creators:
IITH CreatorsORCiD
Badhulika, SushmeeUNSPECIFIED
Item Type: Article
Uncontrolled Keywords: Adenine, Guanine, Pencil, Molybdenum disulfide, Calf-thymus DNA
Subjects: Electrical Engineering
Divisions: Department of Electrical Engineering
Depositing User: Team Library
Date Deposited: 08 Jan 2019 10:02
Last Modified: 08 Jan 2019 10:02
URI: http://raiithold.iith.ac.in/id/eprint/4676
Publisher URL: http://doi.org/10.1016/j.bios.2018.08.055
OA policy: http://www.sherpa.ac.uk/romeo/issn/0956-5663/
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