A, Ashok and V, Lendekar and Devarai, Santhosh Kumar
(2017)
A technique for the usage of agricultural solid substrate extract for the production of L-asparaginase by immobilized Ca-alginate beads.
In: Food, Pharmaceutical and Bioengineering Division 2017 - Core Programming Area at the 2017 AIChE Annual Meeting, 29 October to 3rd November 2017, United States.
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Abstract
Immobilization is a technique used to prevent the contamination of active enzymes, increase substrate mobility and to prevent cell washout. Solid state fermentation (SSF) has been always observed as an alternative to Submerged fermentation (SmF). Optimization of the parameters will help in further improvement. Hie primary enzyme being produced is L-asparaginase that is used as an anti-neoplastic and anti-carcinogenic material. Different solid substrate extracts were tested and the best out of it was selected as wheat bran extract, the activity of crude enzyme is expected to be around 11.05 U/ml in case of the extract media as opposed to 4-5 U/gds in the solid media. In a first of its kind study, an extract of the solid substrate was obtained using buffer solution and this extract is treated as liquid media, SmF process is run using calcium alginate beads as the immobilized material.
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