Development of Invitro 3D Bronchi Model Using Novel Decellularized Smooth Muscle Matrix

Yeleswarapu, Sriya and Pati, Falguni (2018) Development of Invitro 3D Bronchi Model Using Novel Decellularized Smooth Muscle Matrix. Masters thesis, Indian Institute of Technology Hyderabad.

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Abstract

Oxygen is the most important necessity for keeping us all alive. Lungs, in our body allows oxygen into our body. Any damage to this vital organ could be fatal. Incidences of tracheal and bronchial injuries are at a rise with increase in the number of road traffic accidents. Apart from traffic accidents, accidents that involve chest crashing, gun wounds, knife penetrations on chest area also damages the respiratory tract causing either blunt or penetrating injuries. Most of the injuries due to blunt trauma encompasses damage or rupture of trachea and mainstem bronchi. Not only injuries, several other conditions like tumors in the airway or congenital malformations like severe respiratory stenosis, bronchomalacia and bronchial atresia etc. also cause tracheobronchial damages. The theraupeutic approaches for all the cases are not well defined and may vary based on the site, extent of lesion and the severity of damage. There are problems associated with the traditional surgical approches and hence researchers are finding and developing new ways to overcome the complications and also to minimize the organ- demand and supply curve. In this thesis, we made an attempt to develop smooth muscle layer of the bronchi which is the part of the major project lab engineered bronchi which structurally and functionally mimics the native bronchi. Herein, we designed and fabricated a bioreactor using 3D printing technology and used this construct for development of smooth muscle layer of the bronchi model. To support the cells in the construct, we decellularized caprine smooth muscle and prepared its hydogel. Mesenchymal stem cells were embedded into the pre-gel and was incorporated into the printed bioreactor. In this process, we also tried to study the differentiation of MSC into smooth muscle cells in the decellularized smooth muscle matrix which stands as novelty in this project.

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