Electrospun polyaniline nanofiber based chemiresistive nanobiosensor platform for DNA Hybridization detection

Tripathy, Suryasnata and Naithani, Arun and Vanjari, Siva Rama Krishna and Singh, Shiv Govind (2017) Electrospun polyaniline nanofiber based chemiresistive nanobiosensor platform for DNA Hybridization detection. In: 16th IEEE SENSORS Conference, ICSENS 2017, 30 October 2017 to 1 November 2017, Scottish Event Campus (SEC)Glasgow; United Kingdom.

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Abstract

In recent times, nanoscale one-dimensional conductors such as nanotubes have been extensively used as high-gain field-effect sensors for monitoring a variety of biological events, including DNA hybridization. Conventionally, DNA hybridization biosensors are based on single stranded nucleotide (ssDNA) probes, which capture the target DNAs via hybridization with complementary sequences. The formation of this hybridized complex can be detected using several transduction principles [1]–[2]. The widely used colorimetric and electrochemical sensing schemes often involve complex chemistry and are predominately labeled approaches. Alternatively, we propose a label-free chemiresistive platform for DNA hybridization detection based on conductive polymer nanofibres wherein, the nanofibers are surface modified with ssDNA probes specific to a target sequence. Chemiresistive transduction utilizes the change in electrical conductivity of the nanofiber to indicate target analyte detection. DNA, being a negatively charged entity, is a suitable candidate for chemiresistive detection as the formation of the hybridized complex would affect the overall surface charge on the nanofibers. It has been previously illustrated that change in surface charge is likely to induce changes in the resulting conductivity of the nanofiber [3]–[4]. In lieu of this, in this work we report a chemiresistive platform derived from conductive polyaniline/poly-ethylene-oxide (PANi/PEO) nanofibers. In our previous communication [5], we have already demonstrated ultrasensitive detection of cardiac biomarkers using SU8/MWCNT nanofiber based chemiresistive devices. As opposed to the epoxy polymer SU8, the PANiIPEO polymer composite is inherently conductive, which eliminates the need of doping to induce conductivity.

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