Nonlithographic Fabrication of Plastic-based Nanofibers Integrated Microfluidic Biochip for Sensitive Detection of Infectious Biomarker

K, Brince Paul and Panigrahi, Asisa Kumar and Singh, Vikrant and Singh, Shiv Govind (2017) Nonlithographic Fabrication of Plastic-based Nanofibers Integrated Microfluidic Biochip for Sensitive Detection of Infectious Biomarker. ACS Applied Materials & Interfaces. ISSN 1944-8244 (In Press)

Full text not available from this repository. (Request a copy)

Abstract

We report fabrication of a fully integrated plastic based microfluidic biochip for biosensing application. The microfluidic channels were fabricated by tune transfer method and integrated with the pre-functionalized sensing platform. This approach to assemble microchannel into pre-functionalized sensing substrate facilitates controlled functionalization and prevents damages on functionalized surface. The sensing platform comprised of a three electrode system, in which the sensing electrode was integrated with antibody immobilized carbon nanotubes-zinc oxide (C-ZnO) nanofibers. Electrospinning technique was used to synthesize C-ZnO nanofibers and the surface of the nanofibers was covalently conjugated with histidine rich protein II antibodies (AntiHRP II) toward detection of infectious malarial specific antigen, namely histidine rich protein II (HRP II). The analytical performance of the fabricated biochip was evaluated by differential pulse voltammetry method. The device exhibited a high sensitivity of 1.19 mA/ g mL-1 /cm2 over a wide detection range (10 fg/mL - 100 µg/mL) with a low detection limit of 7.5 fg/mL towards HRP II detection. This fully integrated biochip offers a promising cost-effective approach for detection of several other infectious disease biomarkers.

[error in script]

IITH Creators: