Oxidized Alginate-Gelatin Hydrogel: A Favorable Matrix for Growth and Osteogenic Differentiation of Adipose-Derived Stem Cells in 3D

Sarker, B and Zehnder, T and Rath, Subha Narayan and Horch, R E and Kneser, U and Detsch, R and Boccaccini, A R (2017) Oxidized Alginate-Gelatin Hydrogel: A Favorable Matrix for Growth and Osteogenic Differentiation of Adipose-Derived Stem Cells in 3D. ACS Biomaterials Science & Engineering, 3 (8-SI). pp. 1730-1737. ISSN 2373-9878

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Abstract

Alginate-based hydrogels are extensively used matrices for cell encapsulation, but they need to be modified to recapitulate chemical, microstructural and mechanical properties of the native extracellular matrix. Like other cell types, mesenchymal stem cells exhibit rounded and clustered morphologies when they are embedded in alginate hydrogels. In this study, we use covalently crosslinked oxidized alginate-gelatin hydrogels to encapsulate human adipose-derived stem cells in order to investigate cell growth, viability and morphology during osteogenic differentiation taking advantage of the different physicochemical properties of this modified alginate-based hydrogel in comparison to pristine alginate hydrogel. We investigate the effect of hydrogel compositions on stem cell behavior in 3D. Higher viability and spreading morphology of encapsulated cells with interconnected networks are observed in high gelatin containing compositions. More filopodial protrusions from multicellular nodules are noticed during osteogenic differentiation in hydrogels having a high amount of gelatin, confirming their suitability for cell encapsulation and bone tissue engineering applications.

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