Jose, Nimmy
(2016)
Isolation and Screening of Glutaminase & Urease Free Novel Fungal Strains for the Production of L-Asparaginase.
Masters thesis, Indian Institute of Technology Hyderabad.
Abstract
L
-
Asparaginase
is an
amidohydrolase
that
catalyzes
the hydrolysis of amino acid
L
-
aspar
a
gine in
to aspartic acid and ammonia. It
is
used in the treatment of Acute
L
ymphoblastic
Leukemia
(ALL
) and some other malignant lymphoid abnormalities
.
It is
also used in food industry to prevent the formation of a
crylamide
, a carcinogenic substance
in carbohydrate rich fried and baked foods.
Naturally
L
-
Asparaginase
is present i
n plants,
animals and microbes but
microorganisms such as bacteria, yeast and fungi are generally
used for the production of
L
-
Asparaginase
as it is difficult to obtain the same from plants
and animals.
It is found that
the
L
-
Asparaginase
from bacteria causes
side effects in humans
including
anaphylaxis and
serious allergic
reactions
which can be fatal in some cases
. To
overcome this, eukaryotic organisms such as fungi can be used for the production of
L
-
Asparaginase
.
Bu
t sometimes the fungi produces L
-
glutaminase and urease enzymes along
with
L
-
Asparaginase
which is difficult to remove in the purification stage. In order to
prevent this fungal strains which
can produce
L
-
Asparaginase
fre
e of L
-
glutaminase and
urease are
isolated from different sources using standard protocols.
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