RecA stimulates AlkB-mediated direct repair of DNA adducts

Gururaj, S and Mohan, M and Nigam, R and Kodipelli, N and Roy, Anindya (2016) RecA stimulates AlkB-mediated direct repair of DNA adducts. Nucleic Acids Research. pp. 1-10. ISSN 0305-1048

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Abstract

The Escherichia coli AlkB protein is a 2-oxoglutarate/Fe(II)-dependent demethylase that repairs alkylated single stranded and double stranded DNA. Immunoaffinity chromatography coupled with mass spectrometry identified RecA, a key factor in homologous recombination, as an AlkB-associated protein. The interaction between AlkB and RecA was validated by yeast two-hybrid assay; size-exclusion chromatography and standard pull down experiment and was shown to be direct and mediated by the N-terminal domain of RecA. RecA binding results AlkB–RecA heterodimer formation and RecA–AlkB repairs alkylated DNA with higher efficiency than AlkB alone.

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IITH Creators:

IITH Creators	ORCiD
Roy, Anindya	http://orcid.org/0000-0001-8561-907X

Item Type:

Article

Additional Information:

We thank Dr Luke A. Selth, Dame Roma Mitchell Cancer Research Laboratories and Adelaide Prostate Cancer Re- search Centre, The University of Adelaide, SA, Australia for editing the manuscript. We thank Dr N. Ganesh (Indian Institute of Science, Bangalore) and Dr K. Gopinath (Uni- versity of Hyderabad, Hyderabad) for reagents and techni- cal help.

Subjects:

Others > Biotechnology

Divisions:

Department of Biotechnology

Depositing User:

Team Library

Date Deposited:

12 Jul 2016 05:42

Last Modified:

05 Dec 2017 05:00

URI:

http://raiithold.iith.ac.in/id/eprint/2527