Studies On Escherichia Coli AlkB Mediated Direct DNA Repair: Molecular Characterization Of RecA And AlkB Interaction

Shivange, Gururaj (2016) Studies On Escherichia Coli AlkB Mediated Direct DNA Repair: Molecular Characterization Of RecA And AlkB Interaction. PhD thesis, Indian Institute of Technology Hyderabad.

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Abstract

DNA damaging alkylating agents are present abundantly in the environment and also produced endogenously.The majority of the DNA adducts caused by such alkylating agents would be in double-stranded DNA. However, single-strand- specific lesions canarise when DNA double helix is temporarily unwound during replication or recombination. The N1 position of purines and the N3 of pyrimidines, which are normally protected from alkylation by base pairing in duplex DNA, can be alkylated in single-stranded DNA. The Escherichia coliAlkB protein is an oxidative demethylase that repairs such alkylatedbases present in single stranded DNA. Although AlkB function was known in great detail, its regulation was poorly characterized. I hypothesized that some proteins might directly interact with AlkB to regulate its function.

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IITH Creators:	IITH Creators ORCiD
Item Type:	Thesis (PhD)
Uncontrolled Keywords:	DNA, Escherichia coli, proteomic analysis, TD492
Subjects:	Others > Biotechnology
Divisions:	Department of Biotechnology
Depositing User:	Library Staff
Date Deposited:	17 Mar 2016 04:33
Last Modified:	17 Mar 2016 04:33
URI:	http://raiithold.iith.ac.in/id/eprint/2238
Publisher URL:
Related URLs:	Google Scholar

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