Unni, H N
(2015)
Automated Electrowetting-Based Digital Microfluidic Chips for Next-Generation Sequencing and Sample Preparation.
In: Advances in Next Generation Sequencing, 16 June 2015.
Abstract
For identifying and characterizing unknown pathogens next-generation sequencing (NGS) technology is a prominent tool, but its usefulness in time-critical biodefense and public health applications is currently limited by the lack of fast, efficient, and reliable automated DNA sample preparation methods like automated multiplexing technique which use for switching the electrode voltage, the deciding threshold voltage, used for clinical diagnostic to protect the cell from damage and avoiding the cross talk between electrodes because of electrostatic effect to maintain droplet on proper track. We efforts to address those limitations to enhance the system performance for digital microfluidic (DMF) platform to function as a fluid distribution hub, which enabling the integration of multiple subsystem modules into an automated NGS library sample preparation system. DMF platform using an Electrowetting on dielectric (EWOD) bead assay to achieve an efficient DNA recovery. EWOD helps in discretizing the flow and hence it is called as Digital microfluidic (DMF). DMF technology offers a platform for Lab-on-a-chip (LoC), which is concerned with the design of micro total analysis system (µTAS) for chemical and biological applications. This emerging technology combines electronics with biology to open new application areas such as point-of-care diagnosis, on-chip DNA analysis, and automated drug discovery
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