Modulation of Escherichia coli AlkB function by single-stranded DNA-binding protein

Pranjal, K (2015) Modulation of Escherichia coli AlkB function by single-stranded DNA-binding protein. Masters thesis, Indian Institute of Technology Hyderabad.

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Abstract

Cells are always get exposed to the external environment and some metabolism also contribute to the change. In the event of changed external or internal environment, the genome gets damaged. If they remain unrepaired it may be toxic for the cells and may cause some alteration/mutation. Organisms are evolved such that they come out of this kind of condition by various DNA repair mechanisms. Escherichia coli AlkB is a kind of DNA repair protein that belongs to the Fe(II) dependent 2-OG (2-oxoglutarate) dioxygenas superfamily and involved in the repair of mainly 1-methyl adenine (1-meA) and 3-methyl cytosine (3-meC). In this study, the interaction of single stranded DNA-binding protein (SSB) and AlkB was tested in-vitro and observed positive for the interaction. The in-vitrorepair activity was performed with 40mer oligo (40mer Adenine and 40mer Cytosine).The in-vitrorepair activity of purified recombinant GST-AlkB is found to be increased significantly in the presence of SSB, while the ABH3 activity was unchanged in the presence and absence of SSB.

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