Mutation of Trp131residue of Hiv-1 Integrase to T131f and Studying its Interactions with Ledgf/P75

Galli, S K (2015) Mutation of Trp131residue of Hiv-1 Integrase to T131f and Studying its Interactions with Ledgf/P75. Masters thesis, Indian Institute of Technology Hyderabad.

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Abstract

Ubc13, an ubiquitin-conjugating enzyme (Ubc), requires the presence of an Ubc variant (Uev) for polyubiquitination. MMS2 (methyl methane sulfonate) and Uev1A has similar sequence homology. The difference in these two variants is 12 Amino acid residues. But these 12 amino acids residues are dictating two different pathways in which one path way leads to innate immunity (Uev1a with UBC13) and other leads to DNA Repair pathway (MMS2 with UBC13). Generating mutations in MMS2 and analyzing the MMS2 mutants with UBC13, followed by ubiquitin assays will help us to understand which amino acid residue is important for the pathway specificity.

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