Mutant generation of Mms2 and structural analysis of modeled Uev1a N-terminus docked to Uev1a-Ubc13-CHIP heterotrimer

Mohandas, M L (2015) Mutant generation of Mms2 and structural analysis of modeled Uev1a N-terminus docked to Uev1a-Ubc13-CHIP heterotrimer. Masters thesis, Indian Institute of Technology Hyderabad.

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Abstract

Mms2, a Ubiquitin-conjugating enzyme variant (UEV) protein acts aco-factor for Ubc13 in DNA damage repair. This signaling is mediated by assembly of Lys-63 linked poly- ubiquitin chains by Ubc13 and Mms2 complex. Uev1A, another UEV in conjugation with Ubc13 forms Lys-63 poly-ubiquitin chains which lead to activation of NF-kB pathway. Hence, in the presence of different cofactors, Ubc13 performs distinct biological functions. Mms2 and Uev1A were found to have a sequence similarity of more than 90%. This project aims at generating five mutants of Mms2 by changing the residues to that of Uev1A and studying their interaction with Ubc13

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