Quantitative phase imaging of cells using White Light Diffraction Phase Microscopy

Kalva, S K (2015) Quantitative phase imaging of cells using White Light Diffraction Phase Microscopy. Masters thesis, Indian Institute of Technology Hyderabad.

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Abstract

Quantitative phase imaging (QPI) techniques are very advantageous compared to the qualitative phase imaging techniques like bright field microscopy, phase contrast microscopy, differential interference contrast microscopy as the QPI techniques render phase information quantitatively whereas qualitative phase imaging techniques yield phase information qualitatively and cannot provide exact phase values. In QPI techniques, there is no need of exogenous contrast agents to stain or tag the specimen. White light diffraction phase microscopy (wDPM) is one of the QPI techniques which is used to quantify the phase information from the samples.

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