Targeted Multifunctional Multimodal Protein-Shell Microspheres as Cancer Imaging Contrast Agents

John, Renu and Nguyen, F T and Kolbeck, K J and Chaney, E J and Marjanovic, M and Suslick, K S and Boppart, S A (2012) Targeted Multifunctional Multimodal Protein-Shell Microspheres as Cancer Imaging Contrast Agents. Molecular Imaging and Biology, 14 (1). pp. 17-24. ISSN 1536-1632

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Abstract

Purpose: In this study, protein-shell microspheres filled with a suspension of iron oxide nanoparticles in oil are demonstrated as multimodal contrast agents in magnetic resonance imaging (MRI), magnetomotive optical coherence tomography (MM-OCT), and ultrasound imaging. The development, characterization, and use of multifunctional multimodal microspheres are described for targeted contrast and therapeutic applications. Procedures: A preclinical rat model was used to demonstrate the feasibility of the multimodal multifunctional microspheres as contrast agents in ultrasound, MM-OCT and MRI. Microspheres were functionalized with the RGD peptide ligand, which is targeted to α vβ 3 integrin receptors that are over-expressed in tumors and atherosclerotic lesions. Results: These microspheres, which contain iron oxide nanoparticles in their cores, can be modulated externally using a magnetic field to create dynamic contrast in MM-OCT. With the presence of iron oxide nanoparticles, these agents also show significant negative T2 contrast in MRI. Using ultrasound B-mode imaging at a frequency of 30 MHz, a marked enhancement of scatter intensity from in vivo rat mammary tumor tissue was observed for these targeted protein microspheres. Conclusions: Preliminary results demonstrate multimodal contrast-enhanced imaging of these functionalized microsphere agents with MRI, MM-OCT, ultrasound imaging, and fluorescence microscopy, including in vivo tracking of the dynamics of these microspheres in real-time using a high-frequency ultrasound imaging system. These targeted oil-filled protein microspheres with the capacity for high drug-delivery loads offer the potential for local delivery of lipophilic drugs under image guidance

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IITH Creators:

IITH Creators	ORCiD
John, Renu	https://orcid.org/0000-0003-3254-2472

Item Type:

Article

Uncontrolled Keywords:

Magnetomotive optical coherence tomography, Ultrasound imaging, Magnetic resonance imaging, Contrast agents, Protein microspheres, Iron oxide, RGD peptide, Alpha(v) beta(3) targeting,

Subjects:

Biomedical Engineering

Divisions:

Department of Biomedical Engineering

Depositing User:

Team Library

Date Deposited:

30 Jan 2015 07:08

Last Modified:

05 Dec 2017 04:26

URI:

http://raiithold.iith.ac.in/id/eprint/1334