Lipid-coated red fluorescent carbon dots for imaging and synergistic phototherapy in breast cancer

Rengan, Aravind Kumar (2023) Lipid-coated red fluorescent carbon dots for imaging and synergistic phototherapy in breast cancer. Photodiagnosis and Photodynamic Therapy, 41. p. 103314. ISSN 1572-1000

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Abstract

The synthesis of carbon dots using plant leaves is a facile and economically viable approach. Here we report the development of lipid-coated red fluorescent carbon dots (LRCDs), a biocompatible and stable nanomaterial, utilizing Clitoria ternatea leaves. The red fluorescent carbon dots (RCDs) were prepared by hydrothermal method, followed by lipid coating using rotary evaporation for imaging-guided phototherapy. RCDs generate heat in tandem with NIR laser irradiation and could therefore be employed as a photothermal agent in cancer therapy. Additionally, the fluorescent nature of RCDs can be utilized in bioimaging. The fabricated RCDs displayed a characteristic fluorescent emission maximum at 672 nm with a shoulder peak at 723 nm. Hydrophobicity is a major drawback associated with the RCDs, which limits their therapeutic efficiency due to poor biodistribution and rapid clearance. To address this limitation, we coated RCDs with soya lecithin to generate hydrophilic LRCDs with better bioavailability and therapeutic effectiveness. Further analysis using MTT assay reveals high biocompatibility and a distinct photothermal ablation potency of LRCDs against L929 and 4T1 cells, respectively. LRCDs could potentially be synthesized on a large scale and used for a variety of applications due to their low-cost, and biocompatibility.

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IITH Creators:

IITH Creators	ORCiD
Rengan, Aravind Kumar	https://orcid.org/0000-0003-3994-6760

Item Type:

Article

Uncontrolled Keywords:

Bioimaging; Clitoria ternatea; Hydrothermal method; Phototherapy; Red fluorescent carbon dots; 4T1 cell line; absorption; animal cell; Article; bioavailability; biocompatibility; breast cancer; cancer therapy; chemical bond; chorioallantoic membrane assay; Clitoria ternatea; controlled study; evaporation; fibroblast; fluorescence imaging; fluorescence intensity; Fourier transform infrared spectroscopy; hydrodynamics; hydrophilicity; hydrophobicity; MTT assay; NCTC clone 929 cell line; nonhuman; particle size; phototherapy; plant leaf; spectrofluorometry; synthesis; triple negative breast cancer; X ray photoemission spectroscopy; breast tumor; female; human; photochemotherapy; phototherapy; procedures; tissue distribution; carbon; lipid; nanomaterial; phosphatidylcholine; carbon; coloring agent; lipid; photosensitizing agent; quantum dot; Breast Neoplasms; Carbon; Coloring Agents; Female; Humans; Lipids; Photochemotherapy; Photosensitizing Agents; Phototherapy; Quantum Dots; Tissue Distribution

Subjects:

Biomedical Engineering
Biomedical Engineering > Biosensors

Divisions:

Department of Biomedical Engineering

Depositing User:

Mr Nigam Prasad Bisoyi

Date Deposited:

24 Aug 2023 09:32

Last Modified:

24 Aug 2023 09:32

URI:

http://raiithold.iith.ac.in/id/eprint/11612