Unmasking of the von Willebrand A-domain surface adhesin CglB at bacterial focal adhesions mediates myxobacterial gliding motility

Sharma, Gaurav (2023) Unmasking of the von Willebrand A-domain surface adhesin CglB at bacterial focal adhesions mediates myxobacterial gliding motility. Science Advances, 9 (8). ISSN 2375-2548

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Abstract

The predatory deltaproteobacterium Myxococcus xanthus uses a helically-trafficked motor at bacterial focaladhesion (bFA) sites to power gliding motility. Using total internal reflection fluorescence and force microscopies, we identify the von Willebrand A domain-containing outer-membrane (OM) lipoprotein CglB as an essential substratum-coupling adhesin of the gliding transducer (Glt) machinery at bFAs. Biochemical and genetic analyses reveal that CglB localizes to the cell surface independently of the Glt apparatus; once there, it is recruited by the OM module of the gliding machinery, a heteroligomeric complex containing the integral OM β barrels GltA, GltB, and GltH, as well as the OM protein GltC and OM lipoprotein GltK. This Glt OM platform mediates the cell-surface accessibility and retention of CglB by the Glt apparatus. Together, these data suggest that the gliding complex promotes regulated surface exposure of CglB at bFAs, thus explaining the manner by which contractile forces exerted by inner-membrane motors are transduced across the cell envelope to the substratum.

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IITH Creators:

IITH Creators	ORCiD
Sharma, Gaurav	http://www.orcid.org/0000-0002-2861-7446

Item Type:

Article

Uncontrolled Keywords:

Adhesins, Bacterial; Bacterial Adhesion; Bacterial Proteins; Focal Adhesions; Lipoproteins; Myxococcales Cell membranes; Refractive index adhesin; bacterial protein; lipoprotein Adhesin; Biochemical analysis; Cell surfaces; Focal adhesions; Force microscopy; Genetic analysis; Myxococcus xanthus; Outer membrane; Power; Total internal reflection fluorescence microscopy bacterium adherence; focal adhesion; metabolism; Myxococcales Lipoproteins

Subjects:

Others > Biotechnology

Divisions:

Department of Biotechnology

Depositing User:

Mr Nigam Prasad Bisoyi

Date Deposited:

18 Aug 2023 11:57

Last Modified:

18 Aug 2023 11:57

URI:

http://raiithold.iith.ac.in/id/eprint/11575