Biomimicking tendon by electrospinning tissue‐derived decellularized extracellular matrix for tendon tissue engineering

Ruhela, Aakanksha and Bhatt, Akshay and Rath, Subha Narayan and Sharma, Chandra Shekhar (2022) Biomimicking tendon by electrospinning tissue‐derived decellularized extracellular matrix for tendon tissue engineering. Journal of Applied Polymer Science. ISSN 0021-8995

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Abstract

Tendon injuries disturb the equilibrium between mobility and stability, resulting in impaired functions and disabilities. Clinically, it is still challenging to regenerate fully functional tendons. Here, by direct electrospinning, we fabricate goat tendon-derived extracellular matrix (tdECM) fibers using polycaprolactone (PCL) as a supporting polymer. We observe that the incorporation of tdECM particles strongly influences the characteristics of the scaffold, such as wettability, water uptake ability, and mechanical properties. The contact angle of the PCL/tdECM scaffold decreases to zero as compared to 122° for only PCL, making the scaffold completely hydrophilic. The water uptake ability increases by 200% by adding tdECM. The Physicochemical properties are evaluated using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Electrospun fibers mimic the natural ECM structure, while tdECM can provide biochemical cues for the human mesenchymal stem cells to adhere to and differentiate. The scaffolds positively influence cell survival, proliferation, and alignment along the scaffolds of the human umbilical cord-derived mesenchymal stem cells (hUMSCs). This study demonstrates the potential of electrospun ECM/polymer as a bioactive scaffold for in situ tendon regeneration. © 2022 Wiley Periodicals LLC.

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IITH Creators:

IITH Creators	ORCiD
Sharma, Chandra Shekhar	https://orcid.org/0000-0003-3821-1471
Rath, Subha Narayan	UNSPECIFIED

Item Type:

Article

Additional Information:

The authors would like to acknowledge the funding agencies for their generous support of this project. The work was funded by the Department of Science and Technology, Government of India, Nano mission project SR/NM/NT/‐1095/2016, ICMR 35/5/2019‐NANO/BMS, ICMR IMP/2018/000785, and the MHRD, Government of India. We would also like to acknowledge Dr. Asit Sahoo for performing TGA.

Uncontrolled Keywords:

bioscaffold; decellularized extracellular matrix; electrospinning; fibers; human mesenchymal stem cells; tendon

Subjects:

Biomedical Engineering
Chemical Engineering

Divisions:

Department of Biomedical Engineering
Department of Chemical Engineering

Depositing User:

Ms Palak Jain

Date Deposited:

22 May 2023 09:45

Last Modified:

22 May 2023 09:45