Laponite–Gelatin Nanofibrous Microsphere Promoting Human Dental Follicle Stem Cells Attachment and Osteogenic Differentiation for Noninvasive Stem Cell Transplantation

Sarviya, Nandini and Basu, Suparna Mercy and Induvahi, Veernala and Giri, Jyotsnendu (2022) Laponite–Gelatin Nanofibrous Microsphere Promoting Human Dental Follicle Stem Cells Attachment and Osteogenic Differentiation for Noninvasive Stem Cell Transplantation. Macromolecular Bioscience (220034). ISSN 1616-5187

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Abstract

Nanofibrous microspheres (NFM) are emerging as prominent next-generation biomimetic injectable scaffold system for stem cell delivery and different tissue regeneration where nanofibrous topography facilitates ECM-like stem cells niches. Addition of osteogenic bioactive nanosilicate platelets within NFM can provide osteoconductive cues to facilitate matrix mediated osteogenic differentiation of stem cells and enhance the efficiency of bone tissue regeneration. In this study, gelatin nanofibrous microspheres are prepared containing fluoride-doped laponite XL21 (LP) using the emulsion mediated thermal induce phase separation (TIPS) technique. Systematic studies are performed to understand the effect of physicochemical properties of biomimicking NFM alone and with different concentrations of LP on human dental follicle stem cells (hDFSCs), their cellular attachment, proliferation, and osteogenic differentiation. The study highlights the effect of LP nanosilicate with biomimicking nanofibrous injectable scaffold system aiding in enhancing stem cell differentiation under normal physiological conditions compared to NFM without LP. The laponite–NFM shows suitability as excellent injectable biomaterials system for stem cell attachment, proliferation and osteogenic differentiation for stem cell transplantation and bone tissue regeneration. © 2022 Wiley-VCH GmbH.

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IITH Creators:

IITH Creators	ORCiD
Giri, Jyotsnendu	https://orcid.org/0000-0002-2313-4912

Item Type:

Article

Additional Information:

This work was financially supported by the Department of Biotechnology, Ministry of Science and Technology, India (BT/RLF Re‐entry/2013), Department of Science and Technology, Ministry of Science and Technology, Science and Engineering Board, India (SB/S3/CE/048/2015 and CRG/2020/005244) and Abdul Kalam Technology Innovation National Fellowship, Indian National Academy of Engineering, India (INAE/121/AKF/37).

Uncontrolled Keywords:

biocompatible; gelatin; injectable stem cell carrier; laponite XL21; nanofibrous microsphere; osteogenic differentiation

Subjects:

Biomedical Engineering

Divisions:

Department of Biomedical Engineering

Depositing User:

Ms Palak Jain

Date Deposited:

22 May 2023 09:47

Last Modified:

22 May 2023 09:47