Terminalia chebula Polyphenol and Near-Infrared Dye-Loaded Poly(lactic acid) Nanoparticles for Imaging and Photothermal Therapy of Cancer Cells

Pebam, Monika and P, Rajalakshmi and Gangopadhyay, Madhusree and Thatikonda, Shashidhar and Rengan, Aravind Kumar (2022) Terminalia chebula Polyphenol and Near-Infrared Dye-Loaded Poly(lactic acid) Nanoparticles for Imaging and Photothermal Therapy of Cancer Cells. ACS Applied Bio Materials, 5 (11). pp. 5333-5346. ISSN 2576-6422

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Abstract

Photothermal/photodynamic therapies (PTT/PDT) are multimodal approaches employing near-infrared (NIR) light-responsive photosensitizers for cancer treatment. In the current study, IR-775, a hydrophobic photosensitizer, was used in combination with a polyphenols (p)-rich ethyl acetate extract from Terminalia chebula to treat cancer. IR-775 dye and polyphenols were encapsulated in a poly(lactic acid) polymeric nanosystem (PpIR NPs) to increase the cell bioavailability. The hydrodynamic diameter of PpIR NPs is 142.6 ± 2 nm and exhibited physical stability. The nanosystem showed enhanced cellular uptake in a lung cancer cell line (A549). Cell cytotoxicity results indicate that PpIR NPs showed more than 82.46 ± 3% cell death upon NIR light treatment compared to the control groups. Both PDT and PTT generate reactive oxygen species (ROS) and cause hyperthermia, thereby enhancing cancer cell death. Qualitative and quantitative analyses have depicted that PpIR NPs with NIR light irradiation have decreased protein expression of HSP70 and PARP, and increased γ-H2AX, which collectively lead to cell death. After NIR light irradiation, the relative gene expression patterns of HSP70 and CDK2Na were also downregulated. Further, PpIR NPs uptake has been studied in 3D cells and in ovo bioimaging in zebrafish models. In conclusion, the PpIR NPs show good cancer cell cytotoxicity and present a potential nanosystem for bioimaging. © 2022 American Chemical Society.

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IITH Creators:

IITH Creators	ORCiD
P, Rajalakshmi	UNSPECIFIED
Thatikonda, Shashidhar	https://orcid.org/0000-0003-2973-2224
Rengan, Aravind Kumar	https://orcid.org/0000-0003-3994-6760

Item Type:

Article

Additional Information:

The authors would like to acknowledge the following funding agencies: SERB-CRG (CRG/2020/005069, DST/TDT/AMT/2017/22 7), MOE IMPRINT (4291), ICMR (grant no. 35/1/2020-GIA/Nano/BMS), and DBT NNT (BT/NNT/28/1386/2017) for research funding. M.P. would like to acknowledge DST/INSPIRE/IF180306. R.J. would like to acknowledge UGC (938/(OBC)(CSIR-UGC NET DEC.2016)) for financial support. A.K.R. would like to thank BME ID grant for financial support. The graphical abstract and were created using biorender.com .

Uncontrolled Keywords:

bioimaging; IR-775 dye; photothermal/photodynamic therapy; polymeric nanoparticles; polyphenols; zebrafish embryo

Subjects:

Electrical Engineering
Biomedical Engineering
Civil Engineering

Divisions:

Department of Biomedical Engineering
Department of Civil Engineering
Department of Electrical Engineering

Depositing User:

. LibTrainee 2021

Date Deposited:

23 Nov 2022 12:08

Last Modified:

23 Nov 2022 12:08