Duraiswamy, Suhanya and Yung, L.Y.L. and Chen, S.L.
(2020)
Spiral microfluidics enhanced isolation of epithelial cells from infected mice urine.
In: 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020, 4-9 October 2020, Virtual, Online.
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Abstract
Spiral microfluidics has been used in this study to isolate infected epithelial cells containing intracellular bacterial communities (IBCs) from other cells (RBCs, WBCs, and bacteria), in urine. Epithelial cells in human urine are 20 μm to 160 μm in size and hence traditional size based spiral microfluidic isolation cannot be used effectively. We isolated ~60% of epithelial cells by combining Dean Flow Fractionation with switched focusing mode at \textgreater 98% purity. We then use the proposed method for the isolation of epithelial cells from infected mice urine and show the presence of IBCs in the isolated cells as a proof of concept. © 2020 CBMS-0001
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| IITH Creators: |
| IITH Creators | ORCiD |
|---|
| Duraiswamy, Suhanya | https://orcid.org/0000-0002-1803-5389 |
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| Item Type: |
Conference or Workshop Item
(Paper)
|
| Uncontrolled Keywords: |
Intracellular Bacterial Communities (IBCs), Spiral microfluidics, Urine Tract Infections (UTI) |
| Subjects: |
Electrical Engineering |
| Divisions: |
Department of Chemical Engineering |
| Depositing User: |
. LibTrainee 2021
|
| Date Deposited: |
23 Nov 2022 07:27 |
| Last Modified: |
23 Nov 2022 07:27 |
| URI: |
http://raiithold.iith.ac.in/id/eprint/11336 |
| Publisher URL: |
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| Related URLs: |
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