Infrared spectroscopy of live cells from a flowing solution using electrically-biased plasmonic metasurfaces

Kelp, Glen and Li, Joy and Lu, Junlan and DiNapoli, Nicholas and Dutta-Gupta, Shourya and et al, . (2020) Infrared spectroscopy of live cells from a flowing solution using electrically-biased plasmonic metasurfaces. Lab on a Chip, 20 (12). pp. 2136-2153. ISSN 1473-0197

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Abstract

Spectral cytopathology (SCP) is a promising label-free technique for diagnosing diseases and monitoring therapeutic outcomes using FTIR spectroscopy. In most cases, cells must be immobilized on a substrate prior to spectroscopic interrogation. This creates significant limitations for high throughput phenotypic whole-cell analysis, especially for the non-adherent cells. Here we demonstrate how metasurface-enhanced infrared reflection spectroscopy (MEIRS) can be applied to a continuous flow of live cell solution by applying AC voltage to metallic metasurfaces. By integrating metasurfaces with microfluidic delivery channels and attracting the cells to the metasurface via dielectrophoretic (DEP) force, we collect the infrared spectra of cells in real time within a minute, and correlate the spectra with simultaneously acquired images of the attracted cells. The resulting DEP-MEIRS technique paves the way for rapid SCP of complex cell-containing body fluids with low cell concentrations, and for the development of a wide range of label-free liquid biopsies. This journal is © The Royal Society of Chemistry.

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IITH Creators:

IITH Creators	ORCiD
Dutta-Gupta, Shourya	https://orcid.org/0000-0001-5680-3847

Item Type:

Article

Additional Information:

This work was performed in part at the Cornell NanoScale Facility, a member of the National Nanotechnology Coordinated Infrastructure (NNCI), which is supported by the National Science Foundation (Grant NNCI-1542081). Chao Liu was supported by Welch Foundation (No. F-1734). Glen Kelp was partially supported by the European Regional Development Fund and the program Mobilitas Pluss (MOBJD522). We thank Rui Wu, MA for visual image data analysis.

Uncontrolled Keywords:

Cell concentrations; Dielectrophoretic forces; FTIR spectroscopy; Infrared reflection spectroscopy; Infrared spectrum; Label-free techniques; Microfluidic delivery; Therapeutic outcomes

Subjects:

Materials Engineering > Materials engineering

Divisions:

Department of Material Science Engineering

Depositing User:

. LibTrainee 2021

Date Deposited:

23 Nov 2022 09:53

Last Modified:

23 Nov 2022 09:53

URI:

http://raiithold.iith.ac.in/id/eprint/11212