Development of label-free gold nanoparticle based rapid colorimetric assay for clinical/point-of-care screening of cervical cancer

Appidi, Tejaswini and Mudigunda, Sushma V. and Kodandapani, Suseela and Rengan, Aravind Kumar (2020) Development of label-free gold nanoparticle based rapid colorimetric assay for clinical/point-of-care screening of cervical cancer. Nanoscale Advances, 2 (12). pp. 5737-5745. ISSN 2516-0230

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Abstract

Cervical cancer is the fourth largest cancer, affecting women across the globe. Rapid screening is of vital importance for diagnosis and treatment of the disease, especially in developing countries with high risk populations. In this paper, we report a simple, novel and rapid approach for qualitative screening of cervical cancer. A label-free colorimetric technique ("C-ColAur") involving the in situ formation of gold nanoparticles (Au NPs) in the presence of clinical samples is demonstrated. The as-formed Au NPs, owing to the sample composition produced a characteristic color that can be used for the qualitative detection of malignancy. We demonstrated the proof of principle using clinical samples (cervical fluid) collected from both cancer affected and healthy individuals. The results of the detection technique, "C-ColAur"when compared with those of the existing conventional diagnostic procedures (i.e. Pap smear or biopsy), showed 96.42% sensitivity. With the detection time less than a minute and with no/minimal sample processing requirements, the proposed technique shows great potential for point-of-care as well as clinical screening of cervical cancer. © The Royal Society of Chemistry.

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IITH Creators:

IITH Creators	ORCiD
Rengan, Aravind Kumar	https://orcid.org/0000-0003-3994-6760

Item Type:

Article

Additional Information:

The authors thank Padma Shri Prof. Anil K. Gupta for his continuous support, encouragement and belief in the project. The authors acknowledge the Innovation Scholars In-Residence program 2018, hosted by Rashtrapathi Bhavan, New Delhi for the valuable suggestions and discussions with expert committees. The authors acknowledge the support of Dr. T. Sub-ramaneshwara Rao, Medical Director and Chief Oncology Surgeon, Basavatarakam Indo-American Cancer Hospital & Research Institute, Hyderabad, India for necessary permissions to carry out the study. The authors are grateful for the continuous support and efforts of Dr. P. Veena, Dr. P. Sujatha and their respective teams, Dept. of Gynecology, Basavatarakam Indo-American Cancer Hospital & Research Institute, Hyderabad. The authors acknowledge Madhuri Sakaray, research scholar, Centre for Nanoscience & Technology, IST, JNTU Hyderabad for FTIR studies. The authors duly acknowledge DBT-SRISTI-GYTI for the award and grant BIRAC SRISTI PMU-2017/012. The authors also acknowledge MHRD IMPRINT (4291), DST-Inspire (DST/INSPIRE/04/2015/000377), DST-AMT (DST/TDT/AMT/2017/227(G)), and DBT-BIRAC (BIRAC/ IKP0866/BIG-14/19), Govt. of India for the research funding. The authors acknowledge the generous nancial support by the Vasudha Foundations, Hyderabad, India. Tejaswini Appidi acknowledges the Dept. of Science & Technology, Govt. of India for the DST-Inspire fellowship (IF160291).

Uncontrolled Keywords:

Colorimetric assays; Diagnostic procedure; Healthy individuals; In-situ formations; Proof of principles; Qualitative screening; Sample composition; Sample processing

Subjects:

Biomedical Engineering

Divisions:

Department of Biomedical Engineering

Depositing User:

. LibTrainee 2021

Date Deposited:

31 Oct 2022 04:42

Last Modified:

31 Oct 2022 04:42

URI:

http://raiithold.iith.ac.in/id/eprint/11103